Influence of Coating and Size of Magnetic Nanoparticles on Cellular Uptake for In Vitro MRI
Cortés-Llanos B, Ocampo SM, de la Cueva L, Calvo GF, Belmonte-Beitia J, Pérez L, Salas G, Ayuso-Sacido Á.
Nanomaterials. 11(11), 2888 (2021).
Iron oxide nanoparticles (IONPs) are suitable materials for contrast enhancement in magnetic resonance imaging (MRI). Their potential clinical applications range from diagnosis to therapy and follow-up treatments. However, a deeper understanding of the interaction between IONPs, culture media and cells is necessary for expanding the application of this technology to different types of cancer therapies. To achieve new insights of these interactions, a set of IONPs were prepared with the same inorganic core and five distinct coatings, to study their aggregation and interactions in different physiological media, as well as their cell labelling efficiency. Then, a second set of IONPs, with six different core sizes and the same coating, were used to study how the core size affects cell labelling and MRI in vitro. Here, IONPs suspended in biological media experience a partial removal of the coating and adhesion of molecules ...